KBG syndrome: videoconferencing and use of artificial intelligence driven facial phenotyping in 25 new patients

30. November, 2022

Lily Guo # 1, Jiyeon Park # 1, Edward Yi # 1, Elaine Marchi 1, Tzung-Chien Hsieh 2, Yana Kibalnyk 3 4, Yolanda Moreno-Sáez 5, Saskia Biskup 6, Oliver Puk 6, Carmela Beger 7, Quan Li 8, Kai Wang 9, Anastassia Voronova 3 4, Peter M Krawitz 2, Gholson J Lyon 10 11 12

Abstract

Genetic variants in Ankyrin Repeat Domain 11 (ANKRD11) and deletions in 16q24.3 are known to cause KBG syndrome, a rare syndrome associated with craniofacial, intellectual, and neurobehavioral anomalies. We report 25 unpublished individuals from 22 families with molecularly confirmed diagnoses. Twelve individuals have de novo variants, three have inherited variants, and one is inherited from a parent with low-level mosaicism. The mode of inheritance was unknown for nine individuals. Twenty are truncating variants, and the remaining five are missense (three of which are found in one family). We present a protocol emphasizing the use of videoconference and artificial intelligence (AI) in collecting and analyzing data for this rare syndrome. A single clinician interviewed 25 individuals throughout eight countries. Participants’ medical records were reviewed, and data was uploaded to the Human Disease Gene website using Human Phenotype Ontology (HPO) terms. Photos of the participants were analyzed by the GestaltMatcher and DeepGestalt, Face2Gene platform (FDNA Inc, USA) algorithms. Within our cohort, common traits included short stature, macrodontia, anteverted nares, wide nasal bridge, wide nasal base, thick eyebrows, synophrys and hypertelorism. Behavioral issues and global developmental delays were widely present. Neurologic abnormalities including seizures and/or EEG abnormalities were common (44%), suggesting that early detection and seizure prophylaxis could be an important point of intervention. Almost a quarter (24%) were diagnosed with attention deficit hyperactivity disorder and 28% were diagnosed with autism spectrum disorder. Based on the data, we provide a set of recommendations regarding diagnostic and treatment approaches for KBG syndrome.

  1. Department of Human Genetics, NYS Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY, 10314, USA.
  2. Institute for Genomic Statistics and Bioinformatics, University Hospital Bonn, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.
  3. Department of Medical Genetics, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, Canada.
  4. Department of Cell Biology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, Canada.
  5. Medical Genetic Unit, Sistemas Genómicos, Valencia, Spain.
  6. CeGaT GmbH, Praxis für Humangenetik Tübingen, Tübingen, Germany.
  7. MVZ Labor Krone GbR, Filialpraxis für Humangenetik, Bielefeld, Germany.
  8. Princess Margaret Cancer Centre, University Health Network, University of Toronto, Toronto, ON, M5G2C1, Canada.
  9. Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Children’s Hospital of Philadelphia, Philadelphia, PA, 19104, USA.
  10. Department of Human Genetics, NYS Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY, 10314, USA. gholsonjlyon@gmail.com.
  11. George A. Jervis Clinic, NYS Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY, 10314, USA. gholsonjlyon@gmail.com.
  12. Biology PhD Program, The Graduate Center, The City University of New York, New York, NY, USA. gholsonjlyon@gmail.com.
#Contributed equally.