Every tumor is unique. Thus, it is essential to know and understand the underlying molecular pathology of a tumor to treat it properly. With comprehensive genomic profiling (CGP), hundreds of genes and cancer-relevant biomarkers can be analyzed simultaneously. The chances of discovering actionable alterations are increased by simultaneously assessing all these genes and biomarkers. With this approach, results might be available faster, and input material of biopsy samples can be saved. One possibility to comprehensively profile a tumor is the TruSight™ Oncology 500 Assay. With this assay, 523 cancer-relevant genes are analyzed for single nucleotide variants (SNVs), insertions and deletions (indels), and copy number variations (CNVs). Furthermore, the cancer-relevant biomarkers tumor mutational burden (TMB) and microsatellite instability (MSI) are assessed. TMB measures the number of somatic mutations in a cancer patient’s tumor and is quantified as mutations per megabase (mut/Mb). MSI indicates failures of the DNA mismatch repair system.
The application areas of TSO500 are manifold and include:
- stratifying patients for the best treatment choice
- identifying patients eligible for clinical trials
- driving clinical research, especially in the area of immune therapy
You can choose between different TSO500 products for comprehensive genomic profiling of your tumor samples.
Our service covers the full project workflow: from expert consulting and comprehensive bioinformatic evaluation to a clear and structured project report. The project report provides insights into sample quality, sequencing parameters, bioinformatic analysis, and results.
CeGaT Is the Best Partner for Sequencing Your Project
Explore Our Product Portfolio for TSO500
We offer different TSO500 products to address a variety of research questions. Each of our products can be supplemented with further services. We are happy to advise you.
TSO500 Tissue | TSO500 ctDNA |
Species | Species |
Sequencing panel | Sequencing panel |
Number of analyzed genes | Number of analyzed genes |
Starting material Fresh frozen tissue, FFPE tissue, isolated DNA | Starting material Whole blood (e.g., Streck® tubes), plasma, other body fluids, isolated DNA |
Sequencing platform | Sequencing platform |
Included deliverables Project report, files in FASTQ, BAM, VCF, JSON, and TSV format | Included deliverables Project report, files in FASTQ, BAM, VCF, JSON, CSV, and TSV format |
Bioinformatics
TSO500 Tissue
The raw sequencing data (FASTQ format) of TSO500 Tissue are automatically processed using the TSO500 pipeline (Illumina). The analysis includes the mapping of the trimmed sequencing data (BAM format), as well as calling and annotation of SNVs and indels (VCF and JSON format). Furthermore, CNVs are called (VCF format), and the TMB and MSI status are evaluated (TSV format). In addition to the data, a project report (PDF format) and a MultiQC report (HTML format) are generated.
TSO500 ctDNA
The raw sequencing data (FASTQ format) of TSO500 ctDNA are automatically processed using the DRAGEN-Pipeline (Illumina). The analysis includes the mapping of the trimmed sequencing data (BAM format), as well as calling and annotation of SNVs and indels (VCF and JSON format). Furthermore, CNVs are called (VCF format), fusions are detected (CSV format), and the TMB and MSI status are evaluated (TSV and JSON format). In addition to the data, a project report (PDF format) and a MultiQC report (HTML format) are generated.
Technical Information
At CeGaT, paired-end sequencing is performed for TSO500 Tissue using 2 x 100 bp. For our TSO500 ctDNA product, paired-end sequencing with 2 x 150 bp is performed. The Illumina sequencing platforms are used for both products. If you require other sequencing parameters, please let us know! We can provide further solutions.
Gene Directory
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Rapid Turnaround Time
Results in typically 15 days
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Highest accuracy for all processes
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Excellent conditions for your project
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Your data remains your ownership
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Do you have a question or are you interested in our service? Feel free to contact us. We will take care of your request as soon as possible.
Start Your Project with Us
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library preparation option, preferred sequencing depth and required bioinformatic analysis level, if possible.
