Located on the surface of T-cells, the T-cell receptor (TCR) is relevant for recognizing antigens presented by the major histocompatibility complex (MHC) molecules on antigen-presenting cells. Through somatic rearrangements, T-cells express a broad range of unique receptors. These highly diverse heterodimers are mostly composed of two subunits, the α and β chains, and a minor percentage of the γ and the δ chains. The TCR α and γ chains are generated by V/J recombination, which arise from random rearrangements of the variable (V) and joining (J) genes. The β and δ chains are generated by V/J/D recombination, which additionally include diversity (D) genes.
Thus, the individual TCR repertoire is shaped by V/J/D recombination. This recombination results in an extremely diverse complementary-determining region 3 (CDR3). This region is an attractive target to assess the overall TCR repertoire diversity, given that it is thought to be unique to each TCR-β variant.
Investigation of the TCR repertoire can
- provide insights into functions of T-cells in immune response, e.g., immunosuppression.
- enable monitoring drug therapies, such as immunotherapies in cancer and the related change in T-cell status.
- improve personalized medicine by tumor-infiltrating T-cell analysis.
We offer TCR Sequencing to investigate the T-cell receptor repertoire. We provide information about diversity, clonality, and changes in the TCR repertoire or single TCR clones (TCR clone tracking) for different time points.