CeGaT offers sequencing of custom-made libraries compatible with Illumina or PacBio systems. We make use of the latest sequencing technologies. Depending on your needs, samples will be sequenced either on Illumina short-read platforms (NovaSeq X Plus, NovaSeq 6000, MiSeq) or the PacBio Sequel IIe long-read system.
Flexible sequencing options are available, specifically tailored for your project. Please refer to our technical information for further details.
CeGaT Is the Best Partner for Sequencing Your Project
Our Commitment to You
Fast Processing
Turnaround time
≤ 15 business days
High Quality
Highest accuracy for all processes
Secure Delivery
Secure provision of sequenced data via in-house servers
Safe Storage
Safe storage of samples and data after project completion
Our Service
We provide a comprehensive and first-class project support from – selecting the appropriate product to evaluating the data. Each project is supervised by a committed scientist. You will have a contact person throughout the whole project.
Our service includes:
- Detailed project consulting
- Product selection tailored to your project
- Detailed bioinformatic evaluation of your data
- Detailed project report with information about sample quality, sequencing parameters, bioinformatic analysis, and results
Benefit from our dedicated support and accredited workflows.
Explore Our Product Portfolio for Ready to Load Sequencing
We offer different Ready to Load Sequencing options to address a variety of research questions. Different systems, flow cells, and sequencing options are available.
NovaSeq X Plus Platform (Illumina)
10B Flow cell |
SR/PE 200 cycles 2000 Gb |
SR/PE 300 cycles 3000 Gb |
SR: single-read; PE: paired-end
NovaSeq 6000 Platform (Illumina)
SP Flow cell | S1 Flow cell | S2 Flow cell | S4 Flow cell |
R/PE 100 cycles 65 Gb 650 million single-reads CPF* | SR/PE 100 cycles 134 Gb 1330 million single-reads CPF* | SR/PE 100 cycles 333 Gb 3335 million single-reads CPF* | SR/PE 200 cycles 1600 Gb 8000 million single-reads CPF* |
SR/PE 200 cycles 134 Gb 650 million single-reads CPF* | SR/PE 200 cycles 266 Gb 1330 million single-reads CPF* | SR/PE 200 cycles 667 Gb 3335 million single-reads CPF* | SR/PE 300 cycles 2400 Gb 8000 million single-reads CPF* |
SR/PE 300 cycles 200 Gb 650 million single-reads CPF* | SR/PE 300 cycles 400 Gb 1330 million single-reads CPF* | SR/PE 300 cycles 1000 Gb 3335 million single-reads CPF* | |
PE 500 cycles 325 Gb 650 million single-reads CPF* |
SR: single-read; PE: paired-end; CPF: clusters passing filter
*In case of PE sequencing, they will be twice as much.
Miseq Platform (Illumina)
Nano flow cell | Micro flow cell | V2 flow cell | V3 flow cell |
SR/PE 300 cycles 0.3 Gb 1 million single-reads CPF* | SR/PE 300 cycles 1.2 Gb 4 million single-reads CPF* | SR/PE 50 cycles 0.75 Gb 12 million single-reads CPF* | SR/PE 150 cycles 3.3 Gb 22 million single-reads CPF* |
PE 500 cycles 0.5 Gb 1 million single-reads CPF* | SR/PE 300 cycles 4.5 Gb 12 million single-reads CPF* | SR/PE 600 cycles 13.2 Gb 22 million single-reads CPF* | |
SR/PE 300 cycles 200 Gb 650 million single-reads CPF* | PE 500 cycles 7.5 Gb 12 million single-reads CPF* |
SR: single-read; PE: paired-end; CPF: clusters passing filter
*In case of PE sequencing, they will be twice as much.
Sequel IIe Platform (PacBio)
SMRT Cell 8M |
Movie Time – 10-30h (dependent on insert size) 3 million HiFi reads Depends on library insert size, movie time, and sample quality. |
Bioinformatics
Depending on the used sequencing platform, the data formats differ. For short-read sequencing projects you will either receive raw sequencing data as BCL files or demultiplexed FASTQ files. If needed, adapter trimming can be performed. For sequencing projects completed on our PacBio system, you will get the HiFi reads as clipped BAM files or FASTQ files.
Technical Information
At CeGaT, sequencing is performed using the Illumina or PacBio sequencing platforms.
For sequencing projects on the Illumina platforms, we accept any compatible library. Illumina index sequencing primers and custom primers may be used during the sequencing process at CeGaT. If you have generated libraries with commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier (UMI), we can offer sequencing modes with customized sequencing parameters. The lengths of read 1 and read 2, as well as the index read lengths can be adapted to your needs.
For conducting HiFi long-read sequencing projects, we accept libraries generated using the SMRTbell prep kit 3.0. If you also want us to perform the SMRTbell library preparation with your ready-to-use DNA samples (sheared/purified DNA), please let us know and we can discuss the options.
Explore our Ready to Load Sequencing product portfolio with various sequencing options for your libraries. Depending on the batch size, required output, and read number specifications, you can choose from our portfolio.
Contact Us
Do you have a question or are you interested in our service? Feel free to contact us. We will take care of your request as soon as possible.
Start Your Project with Us
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library preparation option, preferred sequencing depth and required bioinformatic analysis level, if possible.
