Ready to Load Sequencing

Use our sequencing capacity for your ready to load libraries

CeGaT offers sequencing of custom-made libraries compatible with Illumina or PacBio systems. We make use of the latest sequencing technologies. Depending on your needs, samples will be sequenced either on Illumina short-read platforms (NovaSeq™ X Plus, NovaSeq™  6000, MiSeq) or the PacBio Sequel IIe long-read system.

Flexible sequencing options are available, specifically tailored for your project. Please refer to our technical information for further details.

CeGaT Is the Best Partner for Sequencing Your Project

High
Flexibility

Scalable for different sample types, quantities, and requirements

Accredited
Procedures

Processing according
to highest quality
standards

Outstanding
Customer Service

Constant scientific supervision of all
project steps

Quality
Made in Germany

In-house processing to maintain our reliable and trusted quality

Our Commitment to You

Fast Processing

Turnaround time
≤ 15 business days

High Quality

Highest accuracy for all processes

Secure Delivery

Secure provision of sequenced data via in-house servers

Safe Storage

Safe storage of samples and data after project completion

Our Service

We provide a comprehensive and first-class project support – from selecting the appropriate product to evaluating the data. Each project is supervised by a committed scientist. You will have a contact person throughout the whole project.

Our service includes:

  • detailed project consulting
  • product selection tailored to your project
  • detailed bioinformatic evaluation of your data
  • detailed project report with information about sample quality, sequencing parameters, bioinformatic analysis, and results

Benefit from our dedicated support and accredited workflows.

Explore Our Product Portfolio for Ready to Load Sequencing

We offer different Ready to Load Sequencing options to address a variety of research questions. Different systems, flow cells, and sequencing options are available.

NovaSeqTM X Plus Platform (Illumina)

10B flow cell

25B flow cell

SR/PE

200 cycles

2000 Gb

SE/PE

300 cycles

8000 Gb

SR/PE

300 cycles

3000 Gb

SR: single-read; PE: paired-end

Single-Lane Sequencing on NovaSeq™ X Plus Platform (Ilumina)

25B flow cell single lane*

PE

2 x 150 cycles
up to 1000 Gb

PE: paired-end
*Available for one or several 25B lanes

NovaSeqTM 6000 Platform (Illumina)

SP flow cell

S1 flow cell

S2 flow cell

S4 flow cell

SR/PE

100 cycles

65 Gb

650 million single reads CPF*

SR/PE

100 cycles

134 Gb

1330 million single reads CPF*

SR/PE

100 cycles

333 Gb

3335 million single reads CPF*

SR/PE

200 cycles

1600 Gb

8000 million single reads CPF*

SR/PE

200 cycles

134 Gb

650 million single reads CPF*

SR/PE

200 cycles

266 Gb

1330 million single reads CPF*

SR/PE

200 cycles

667 Gb

3335 million single reads CPF*

SR/PE

300 cycles

2400 Gb

8000 million single reads CPF*

SR/PE

300 cycles

200 Gb

650 million single reads CPF*

SR/PE

300 cycles

400 Gb

1330 million single reads CPF*

SR/PE

300 cycles

1000 Gb

3335 million single reads CPF*

PE

500 cycles

325 Gb

650 million single reads CPF*

SR: single-read; PE: paired-end; CPF: clusters passing filter
*In case of PE sequencing, they will be twice as much.

Miseq Platform (Illumina)

Nano flow cell

Micro flow cell

V2 flow cell

V3 flow cell

SR/PE

300 cycles

0.3 Gb

1 million single reads CPF*

SR/PE

300 cycles

1.2 Gb

4 million single reads CPF*

SR/PE

50 cycles

0.75 Gb

12 million single reads CPF*

SR/PE

150 cycles

3.3 Gb

22 million single reads CPF*

PE

500 cycles

0.5 Gb

1 million single reads CPF*

SR/PE

300 cycles

4.5 Gb

12 million single reads CPF*

PE

600 cycles

13.2 Gb

22 million single reads CPF*

PE

500 cycles

7.5 Gb

12 million single reads CPF*

SR: single-read; PE: paired-end; CPF: clusters passing filter
*In case of PE sequencing, they will be twice as much.

Sequel IIe Platform (PacBio)

SMRT Cell 8M

Movie time – 10-30 h
3 million HiFi reads

Dependant on library insert size, movie time, sample quality.

Bioinformatics

Depending on the used sequencing platform, the data formats differ. For short-read sequencing projects you will either receive raw sequencing data as BCL files or demultiplexed FASTQ files. If needed, adapter trimming can be performed. For sequencing projects completed on our PacBio system, you will get the HiFi reads as clipped BAM files or FASTQ files.

Our Workflow

Hier finden Sie den gesamten Prozessablauf

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Support & Offer

Support &
Offer

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Sample Receipt & Quality Control

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Library Preperation & Sequencing

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Bioinformatic Analysis & Data Delivery

Technical Information

At CeGaT, sequencing is performed using the Illumina or PacBio sequencing platforms.

For sequencing projects on the Illumina platforms, we accept any compatible library. Illumina index sequencing primers and custom primers may be used during the sequencing process at CeGaT. For Single-Lane Sequencing, custom primers are not available. If you have generated libraries with commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier (UMI), we can offer sequencing modes with customized sequencing parameters. The lengths of read 1 and read 2, as well as the index read lengths can be adapted to your needs.

For conducting HiFi long-read sequencing projects, we accept libraries generated using the SMRTbell prep kit 3.0. If you also want us to perform the SMRTbell library preparation with your ready-to-use DNA samples (sheared/purified DNA), please let us know and we can discuss the options.

Explore our Ready to Load Sequencing product portfolio with various sequencing options for your libraries. Depending on the batch size, required output, and read number specifications, you can choose from our portfolio.

Downloads

Ready to Load Sequencing Flyer (EN)
Single-Lane Sequencing (EN)
NovaSeq™ X Plus Performance

Contact Us

Do you have a question or are you interested in our service? Feel free to contact us. We will take care of your request as soon as possible.

Start Your Project with Us

We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.

When getting in contact, please specify sample information including starting material, number of samples, preferred library preparation option, preferred sequencing depth and required bioinformatic analysis level, if possible.