The 16S ribosomal RNA (rRNA) gene is approximately 1.5 kb long and contains several conserved and hypervariable regions (V1-V9) that vary between different bacteria. These hypervariable sequences can be used to identify and characterize microbial diversity. Therefore, the 16S rRNA gene is a common marker to characterize microbial communities in various specimens.
Using the PacBio single molecule real-time (SMRT) technology, we accurately sequence the full-length 16S rRNA gene, covering all variable regions with an average HiFi read length of about 1.5 kb. The extraordinary accuracy and length of PacBio HiFi reads generated using circular consensus sequencing (CCS) mode allows microbial taxa detection at high resolution.
Applications of full-length 16S sequencing are diverse and include:
- Characterization of different microbial communities
- Microbial biomarker detection
- Disease monitoring
- Drug development
Our Full-Length 16S Sequencing product provides interesting insights for diverse research questions.
CeGaT Is the Best Partner for Sequencing Your Project
Our Commitment to You
Fast Processing
Turnaround time
≤ 15 business days
High Quality
Highest accuracy for all processes
Secure Delivery
Secure provision of sequenced data via in-house servers
Safe Storage
Safe storage of samples and data after project completion
Our Service
We provide a comprehensive and first-class project support from – selecting the appropriate product to evaluating the data. Each project is supervised by a committed scientist. You will have a contact person throughout the whole project.
Our service includes:
- Detailed project consulting
- Product selection tailored to your project
- Detailed bioinformatic evaluation of your data
- Detailed project report with information about sample quality, sequencing parameters, bioinformatic analysis, and results
Benefit from our dedicated support and accredited workflows.
Explore Our Product for Full-Length 16S Sequencing
We offer Full-Length 16S Sequencing to address a variety of research questions. Would you like to have bioinformatic analyses performed on your data in addition to the included deliverables? Our product can be supplemented with further services. We are happy to advise you.
Bioinformatics
Raw sequencing data are automatically processed. We offer different levels of bioinformatic analysis. The default level is Level 1. With increasing bioinformatic level, more data are delivered. The higher level includes the data from the lower level. In addition to the data, and independent of the analysis level, a project report is generated.
Level 1:
- Demultiplexing of the sequencing data (HiFi reads, FASTQ format)
Level 2:
- Taxonomic classification down to species level (if possible)
- Mapping of the sequencing data against microbiome database (FASTA, RMA)
- Generation of taxonomic tables including sequence counts and relative abundances (XLSX, TSV, and BIOM format)
- Generation of barplots showing relative abundances (PNG format)
Technical Information
At CeGaT, sequencing is performed using the PacBio Sequel IIe platform. If you require other sequencing parameters, please let us know! We can provide further solutions.
We are pleased to offer you our specifically designed stool sampling kit, which provides convenient and unbiased self-collection of fecal material combined with our DNA isolation service. Please also reach out to us to discuss sampling and DNA extraction of various starting material.
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Contact Us
Do you have a question or are you interested in our service? Feel free to contact us. We will take care of your request as soon as possible.
Start Your Project with Us
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library preparation option, preferred sequencing depth and required bioinformatic analysis level, if possible.
