DNA Sequencing
Please quantify the concentration of your sample using a fluorometric based method (e.g., Qubit). The quality of your samples should be further assessed by using the Fragment Analyzer or by detecting the optical density. We recommend using samples with high molecular weight DNA that were RNase treated resulting in an OD 260/280 of 1.8 – 2.0. Of course, also fragmented DNA can be sequenced when a sequencing library is prepared according to our specially selected or adapted protocols. All isolated DNA samples should be delivered in molecular biology grade water, 10 mM Tris-HCl pH 8 or Elution Buffer, free of EDTA.
We ask you to send samples in 2 ml screw cap tubes from Sarstedt, or in 1.5 ml Eppendorf tubes. For a sample size ≥ 16 samples, please provide the samples in Eppendorf twin.tec PCR Plates 96, skirted.
Please contact us for further information about submitting lower input amounts or degraded sample material.
RNA Sequencing
Please quantify the concentration of your sample using a fluorometric based method (e.g., Qubit). The quality of your sample should be further assessed by using the Bioanalyzer or by detecting the optical density, resulting in an OD 260/280 ~ 2.0. RNA samples need to be DNase treated. All RNA samples should be delivered in molecular biology grade water, 10 mM Tris-HCl pH 8 or Elution Buffer, free of EDTA.
We ask you to send samples in 2 ml screw cap tubes from Sarstedt, or in 1.5 ml Eppendorf tubes. For a sample size ≥ 24 samples, please provide the samples in Eppendorf twin.tec PCR Plates 96, skirted.
For Single-Cell RNA Sequencing, we require frozen single cell suspensions. Each sample should contain 1 million cells in 1 ml cryopreservation medium with a cell viability > 90%. No large cell aggregates should be present in the cell suspension prior to cryopreservation. Please provide the samples in Eppendorf twin.tec PCR Plates 96, skirted.
Please contact us for further information about submitting lower input amounts or degraded sample material.
Ready to Load Sequencing
Read to Load Sequencing libraries for the Illumina platforms need to be diluted in 10 mM Tris-HCl, pH 8.5. PacBio libraries need to be delivered in PacBio elution buffer. When we shall use your custom read primers for the sequencing of your library, please send 30 µl of 100 µM concentrated primer.
Samples for DNA and RNA Isolation
General Sample Shipment Recommendations
When sending your samples for sequencing, please consider the following shipment conditions:
- DNA samples should be shipped chilled at 4° C or frozen.
- RNA samples should be shipped frozen on dry ice.
- Sequencing libraries should be shipped frozen on dry ice.
Contact Us
Do you have a question or are you interested in our service? Feel free to contact us. We will take care of your request as soon as possible.
Start Your Project with Us
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library preparation option, preferred sequencing depth and required bioinformatic analysis level, if possible.
