A gene panel covers a distinct set of regions within the genome. The selected set of genes is enriched, and only these targets will be sequenced by next-generation sequencing. With this method, genes of interest can be examined in many samples simultaneously with high sequencing depth and a reduced percentage of underrepresented regions. As a result, you benefit from more manageable data sets for the subsequent analysis.
We have been using the concept of panel sequencing in genetic diagnostics since 2009. Based on our long-standing expertise, we can offer our predesigned CeGaT panels for your research project or assist you in a custom panel design with subsequent panel validation.
CeGaT Is the Best Partner for Sequencing Your Project
Our Commitment to You
Fast Processing
Turnaround time
≤ 15 business days
High Quality
Highest accuracy for all processes
Secure Delivery
Secure provision of sequenced data via in-house servers
Safe Storage
Safe storage of samples and data after project completion
Our Service
We provide a comprehensive and first-class project support – from selecting the appropriate product to evaluating the data. Each project is supervised by a committed scientist. You will have a contact person throughout the whole project.
Our service includes:
- Detailed project consulting
- Product selection tailored to your project
- Detailed bioinformatic evaluation of your data
- Detailed project report with information about sample quality, sequencing parameters, bioinformatic analysis, and results
Benefit from our dedicated support and accredited workflows.
Explore Our Product Portfolio for Panel Sequencing
We offer different Panel Sequencing (PAS) products to address a variety of research questions. Would you like to have bioinformatic analyses performed on your data in addition to the included deliverables? Each of our products can be supplemented with further services. We are happy to advise you.
Bioinformatics
Raw sequencing data are automatically processed. We offer different levels of bioinformatic analysis. The default level is Level 1. With increasing bioinformatic level, more data are delivered. All higher levels include the data from the lower levels. In addition to the data, and independent of the analysis level, a project report is generated.
Level 1:
- Demultiplexing and adapter trimming of the sequencing data (FASTQ format)
Level 2:
- Mapping of the sequencing data (BAM format)
Level 3:
- Calling of single nucleotide variants (SNV) and small insertions and deletions (indels) (VCF format)
Level 4:
- Annotation of the SNVs and indels (TSV format)
- Calling of large homozygous deletions (TSV format)
Level 5:
- Variant comparison in multiple samples (TSV and PDF format)
- Cancer somatic mutation analysis (tumor and normal sample required) based on exome sequencing data (TSV and PDF format)
- HLA typing based on exome sequencing data (TSV and PDF format)
Comparison of affected genes between multiple samples can be offered upon request, as well as other statistical evaluations.
If you design a custom panel with us, the data format for delivery and the individual data analysis will be defined according to your needs.
Technical Information
When you choose one of our predesigned CeGaT panels, you can rely on our expertise in sequencing. The CeGaT panel design is based on scientific evaluation of the respective disease, and panels are updated periodically based on the latest scientific knowledge.
We support you in defining the target and validating your custom panel following the CAP and ISO:15189 quality standards approved for diagnostics.
We suggest paired-end sequencing of CeGaT panel-enriched libraries (2 x 100 bp) using the Illumina sequencing platforms. If you require other sequencing parameters, please let us know! We can provide further solutions.
Further Information about Panel Sequencing
Panel sequencing is a cost-effective way to investigate diseases, such as Mendelian diseases or cancer. With the predefined panels, specific genetic regions are targeted and enriched. These regions are associated with the phenotype or disease of interest. Thus, various different panels are available at CeGaT.
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Contact Us
Do you have a question or are you interested in our service? Feel free to contact us. We will take care of your request as soon as possible.
Start Your Project with Us
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library preparation option, preferred sequencing depth and required bioinformatic analysis level, if possible.
