Comparing the Performances of Our NovaSeq™ Instruments

July 28, 2023

The NovaSeq™ X Plus instrument employs the same general principles for sequencing libraries of short DNA molecules as the NovaSeq™ 6000 instrument. However, there are differences in the sequencing chemistry (e.g., modified nucleotides, polymerases) and the image acquisition system. These could impact the obtained sequencing data.

We conducted several tests with different datasets and specifications to show that the sequencing results are comparable between the NovaSeq™ X Plus and the NovaSeq™ 6000. Subsequently, we compared the sequencing data with the following results:

  • The NovaSeq™ X Plus instrument has a lower reported error rate than the NovaSeq™ 6000 instrument.
  • The NovaSeq™X Plus instrument does not introduce sequencing errors that negatively affect the sensitivity and precision of variant calling compared to the NovaSeq™ 6000 instrument.
  • No difference can be detected between the sequence error profiles of the NovaSeq™ X Plus instrument and the NovaSeq™ 6000 instrument.

The NovaSeq™ X Plus instrument does not add bias due to sequence or GC content. Read sequences accurately reflect the molecular sequences in the libraries, allowing for highly accurate variant calling. Overall, the NovaSeq™ X Plus sequencer can replace the currently used NovaSeq™ 6000 instrument from a technical point of view.

Find here more information about the sequencing technologies used by CeGaT